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英文名字 Mature BDNF/proBDNF Combo Rapid ELISA Kit: Human, Mouse, Rat
中文名称 Mature BDNF/proBDNF Combo Rapid ELISA Kit:人,小鼠,大鼠
供应商 Biosensis
产品货号 BEK-2240
产品报价 ¥9193.8/1set
购买方式 Biosensis中国区现货中心已经建立,大部分产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
产品新闻
背景资料 FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity).
产品描述 The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance.
Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples.
产品特点 该ELIAS试剂盒包含:2 x 96孔预包被条孔板(可拆卸) (1 x BDNF antibody, 1 x proBDNF antibody), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
保存建议 厂家推荐冰袋运输。厂家推荐试剂盒保存于4 °C
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其他 Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。Biosensis的产品系列不仅包括持续增长的神经科学研究抗体系列,还包括200多种定量研究ELISAs试剂盒(1板或2板,自由选择),组织染色(退化神经元染色试剂盒、病理髓鞘染色试剂盒、淀粉样斑块染色试剂等)和细胞可视化试剂(染色)以及针对神经科学和细胞疾病研究的纯化的蛋白质。我们的抗体和试剂已被广泛应用于各种技术,包括蛋白质印迹,免疫组织化学,流式分析,共聚焦显微镜实时成像,生物抑制和细胞培养以及克隆。

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